Co-culture

Co-culture was commonly used in 90s as a helpful method in group of patients who had repeated failure of test tube baby and poor quality of embryos or slow. The cells form the future mother’s uterus are kept alive in the laboratory environment, and developing embryos live in a medium composed of these cells. With this method which is also known as artificial uterus, some substances secreted by the cells of mother’s uterus are considered to have positive effects on development of an embryo.  

With endometrial co-culture method which is the most frequently used method, a tissue sample is collected by a cannula from the walls of the uterus (endometrium) of the patient at the 21st day of menstruation. This tissue is either frozen and stored for future procedures or cultured for immediate use. Tissue sample is stored in special medium in the laboratory environment and used to form an artificial endometrium. Then the embryos are transferred onto this artificial uterus tissue to be cultured. In the meantime, some substances secreted by uterus cells are intended to feed the embryos as in their natural environment and to develop in a better quality. Presence of other substances removing hazardous residuals from the periphery of the embryo has been shown in addition to co-culture environments containing materials required for an embryo to develop. The achieved embryos are often transferred between the 5th and 6th days, which is called blastocyst phase.

Activity studies show that the co-culture does not provide a significant increase in the rate of pregnancy and implantation compared to the new mediums in today’s world where complex successive mediums are used to support development of an embryo up until 5th or 6th day. Thus, co-culture is not a method used commonly because its effectiveness has not been demonstrated.

Activity studies show that the co-culture does not provide a significant increase in the rate of pregnancy and implantation compared to the new mediums in today’s world where complex successive mediums are used to support development of an embryo up until 5th or 6th day. Thus, co-culture is not a method used commonly because its effectiveness has not been demonstrated.

Do you want to keep informed about us?
florence nightingale hastanesi

Copyright 2016 Group Florence Nightingale Hospitals. All rights reserved.

The information on this website is not intended to replace any medical advice given by physicians with access to your detailed medical history.